Download Meningococcal Disease: Methods and Protocols (Methods in by Andrew J. Pollard, Martin C.J. Maiden PDF

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By Andrew J. Pollard, Martin C.J. Maiden

Because the first popularity of outbreaks of cerebrospinal or noticed fever on the finish of the 19th and the start of the 20th centuries, the threat of the meningococcus has been excessive at the record of public overall healthiness prio- ties. Few if any pathogens surpass the meningococcus within the rapidity and sev- ity with which it devastates formerly fit contributors. The problem of realizing the biology of this interesting microbe is titanic, yet few will doubt that profitable keep an eye on of meningococcal meningitis and septicemia will simply transpire throughout the program of a physique of impressive unique inf- mation, together with key trivia of its molecular biology. within the first of 2 spouse volumes, Meningococcal Vaccines, the workforce of specialists amassed through Andy Pollard and Martin Maiden converge to p- vide a magnificent accumulation of molecular instruments with which to put naked the secrets and techniques of the meningococcus. the result of this bold and welcome v- ume symbolize an exhilarating and lots more and plenty wanted source for we all within the box.

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Culture a. Chocolate agar b. Anaerobic blood-agar plate for anaerobic incubation c. Broth inoculation Consider antigen detection Consider DNA detection Turbid CSF (no centrifugation) 1. Microscopy make 3–4 slides for a. Gram staining b. Acridine orange-staining c. Slide for teaching/extra 2. Culture a. Chocolate agar b. Anaerobic blood-agar plate for anaerobic incubation c. Broth inoculation d. Consider direct inoculation for antibiotic sensitivity testing e. Consider optochine test (on blood-agar medium) when suspecting pneumococci Consider antigen detection Consider DNA detection sistent suspicion of meningococcal disease.

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Rep. Rev. 7, R55–R91. 2. Chief Medical Officer, Chief Nursing Officer, Chief Pharmaceutical Officer (1999) Introduction of Immunisation Against Group C Meningococcal Infection. Department of Health, London. 3. , and Skold, O. (1991) Rapid diagnosis of meningococcal meningitis by polymerase chain reaction. Lancet 337, 1568–1579. 4. Saunders, N. , Zollinger, W. , and Rao, V. B. (1993) A rapid and sensitive PCR strategy employed for amplification and sequencing of porA from a single colony forming unit of Neisseria meningitidis.

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